Preclinical evaluation of antidiabetic activity of poly herbal plant extract in streptozotocin induced diabetic rats p. In this study, body weight, blood glucose level, glycated hemoglobin, lipid profile, liver function tests, and renal function tests were performed in animal models. Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase abstract importance of the field. Evaluation of in vitro anti diabetic activity of selected. Aqueous extracts of zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Evaluation of in vitro anti diabetic activity of selected plant extracts sindhu. The extract was subjected to detail in vivo antidiabetic study. Wagh 1, manoj mohan 2 and at shivashanmugam 2 1ibss college of pharmacy, malkapur ms 2srips cop, coimbatore. Both extracts showed a significant antidiabetic activity. In vitro investigation of antidiabetic potential of selected.
The antidiabetic activity of the plant extract was tested against streptozotocin induced toxicity in male albino rats. To the best of the authors knowledge, there are no previous scientific reports on the antidiabetic activity of the leaf latex of a. A medicinal plant used by canadian james bay cree nations to treat symptoms related to diabetes in doaj. Antidiabetic activity of extracts of anacardium occidentale. Frontiers in vitro and in vivo antidiabetic properties. Eighteen recipes maximum 2 plants were selected after an ethnobotanical survey of medicinal plant recipes used by tmps in the management of diabetes mellitus in the 6 geopolitical zones of. The increasing prevalence of type 2 diabetes mellitus and the negative clinical outcomes observed with the commercially available antidiabetic drugs have led to the. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 gl.
Medicinal plants with potential antidiabetic activity a. Dec 19, 2019 in vitro antidiabetic activity by glucose uptake method. May 02, 2017 treatment with the antidiabetic drug, glibenclamide 10 mgkg body weight lowered blood glucose level by 51. Department of microbiology, kakatiya university, warangal, india. Plant material was subjected to the extraction preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform, petroleum ether and hexane. The plant showed significant inhibition activity, so further the compound isolation, purification, and characterization which is responsible for inhibiting activity, has to be done for the usage of antidiabetic agent. There was a dosedependent increase in percentage inhibitory activity against alphaamylase enzyme. Pdf models to study in vitro antidiabetic activity of. In vitro and in vivo evaluation of the antidiabetic effect. All extracts showed significant antioxidant activity in correlation to phytoconstituents of the extracts. Further studies to investigate the mechanism of action for hypoglycemic activity for these plant species should be done in order to explore possibilities of developing a drug that can function by similar mode of action as the plant extract.
Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic effect and phytochemical screening of the aqueous and 70% ethanol extracts on alloxaninduced diabetic mice. In vitro investigation of antidiabetic potential of. In vivo antidiabetic activity of the ethanolic crude. In vitro antidiabetic activity of cardiospermum halicacabum leaves extracts. Identification and quantification of bioactive compounds present was analysed by hplc. Pdf in vivo antidiabetic activity of the aqueous leaf extract of. Antidiabetic activity of the compounds isolated from. Antidiabetic activity and phytochemical screening of. Information about the openaccess article in vivo antidiabetic activity of the ethanolic crude extract of sorbus decora c. In vivo assessment of antidiabetic and antioxidant activities. Frontiers in vitro and in vivo antidiabetic properties of. In vitro free radical scavenging and antidiabetic activity of. Their antioxidant activities were measured by 2,2diphenyl1picrylhydrazyl dpph free radical scavenging.
Both extracts were investigated for their inhibitory effect on. Further studies for investigating the specific compounds responsible for such beneficial role in diabetes would open new outlook in the therapy of type 2 diabetes. Alpha glucosidase inhibiting activity and in vivo antidiabetic activity of fraxinus floribunda bark in streptozotocininduced diabetic rats arunika subba 1, ram kumar sahu 2, shiv bhardwaj 3, palash mandal 1 1 department of botany, plant physiology and pharmacognosy research laboratory, university of north bengal, siliguri, west bengal, india 2 department of pharmacy, pt. Ethanol extract of leaves of tn was found to stimulate the glucose uptake in c2cl2 muscle cells by 61. The in vitro antidiabetic activity of the aqueous leaf extract and agnps was confirmed by non. Ajuga remota, antidiabetic activity, blood glucose level, diabetes mellitus, glibenclamide. In vitro free radical scavenging and antidiabetic activity. The present study was designed to investigate the glucose uptake of antidiabetic activity crude nhexane,ethanol, methanol and aqueous leaf extracts of cardiospermum halicacabum. More number of plant and plant products have been scientifically tested and reported to posses anti diabetic activity 2635 examples of herbal medicines with proven anti diabetic activity plant part used. After acute toxicity test, the swiss albino mice were induced with alloxan to. In vitro and in vivo antidiabetic effect of extracts of melia. The aim of the current study was to investigate the antidiabetic effects of ethanol extract of leaves of a.
In vitro and in vivo antidiabetic effect of extracts of. Pdf on nov 11, 2015, phillip ogola and others published in vivo antidiabetic activity of the aqueous leaf extract of croton macrostachyus in. The antioxidant activity of plant extract was analysed using 2,2diphenyl1picrylhydrazyl dpph, superoxide anion scavenging activity, hydroxyl radical scavenging assay and reducing power analysis methods. The present study was aimed to evaluate the antidiabetic and in vivo antioxidant property of the leaves of blumea balsamifera in streptozotocin stz induced diabetic rats. A higher antiinflammatory activity was observed in extract obtained from microwave extraction 64. Models to study in vitro antidiabetic activity of plants. These results demonstrate significant antidiabetic potential of the ethanol extract of leaves of a. The present study was designed to investigate the glucose uptake of antidiabetic activity crude nhexane,ethanol, methanol and aqueous leaf.
The aqueous plant extract exhibited a dose dependent hypoglycemic activity in alloxanized diabetic mice. In vitro and in vivo evaluation of the antidiabetic effect of. In india, its leaves are traditionally used for diabetes mellitus. The selected plant parts were subjected to solvent extraction and analyzed for their antidiabetic activity using in vitro assays such as.
Where acute toxicity study was carried out using 30 albino mice, randomly assigned into six groups of five mice each. Evaluation of in vitro antidiabetic activity of selected plant extracts. A medicinal plant used by canadian james bay cree nations to treat symptoms related to diabetes. Acute toxicity and antidiabetic activity of asystacia. Pdf on nov 11, 2015, phillip ogola and others published in vivo antidiabetic activity of the aqueous leaf extract of croton macrostachyus in alloxan induced diabetic mice find, read and cite.
Antidiabetic activity and phytochemical screening of extracts. Treatment with the antidiabetic drug, glibenclamide 10 mgkg body weight lowered blood glucose level by 51. For in vivo studies, blood glucose level was monitored at different intervals after administration of varying doses of the extract for its hypoglycemic 1006000 mgkg b. To study and compare the effect of poly herbal plant extract phpe with glibenclamide gl on various parameters in streptozotocin stz induced diabetic rats. Invitro and invivo studies of antidiabetic activity of. The main objective of this work is to evaluate the in vitro anti diabetic activity of methanolic extracts of cinnamomum zeylanicum, piper betle, artocarpus heterophyllus and artocarpus altilis. The present study was aimed to investigate in vivo, in vitro antidiabetic activity of aqueous extract of terminalia paniculata bark aetpb and characterize its possible phytoconstituents responsible for the actions. In the present study, the whole plant was selected in case of herbs, leaves and stem in case of shrubs and leaves in case of trees on the basis of availability of phytoconstituents. In vivo assessment of antidiabetic and antioxidative.
The present investigation includes the preliminary screening of phytochemicals and evaluation of in vitro antidiabetic activity of psidium guajava leaves extracts. In vitro antidiabetic activity of caesalpina digyna r. This extract was used for in vitro and in vivo analysis of antidiabetic activity. According to the recent findings 14, 15, substances with cytotoxic activities are therapeutically recommended for antidiabetic activity. Asian journal of plant science and research, 2011, 1 2. Sample powders of each plant were soaked in ethanol 5. Plant lectins are proteins bind to specific carbohydrate groups on the plasma membranes of mammalian cells, inducing variety of biological effects 9. The in vitro antidiabetic activity of petroleum ether nonpolar and ethanol polar extracts were evaluated in c2c12 cell lines by 34,5dimethylthiazol2yl2,5diphenyltetrazolium bromide assay cell viability method and glucose uptake assay. Phytochemical and in vitro antidiabetic activity of psidium. Further, the antidiabetic activity of the extracts was compared with standard marketed antidiabetic drugs insulin and metformin table 4 and fig. Antidiabetic and antihyperlipidemic activities of the leaf.
In vivo antidiabetic activity of aqueous extract of. Evaluation of the antidiabetic property of aqueous leaves. In vitro studies on alpha amylase and alpha glucosidase. Phytochemical evaluation and in vitro antidiabetic. In vitro antidiabetic activity of cardiospermum halicacabum leaves extracts by stalin. In vitro and in vivo antidiabetic and antioxidant activities of methanolic leaf. Evaluation of in vivo antidiabetic, antidyslipidemic and in. Toxicity signs and mortality were observed in the rats within a period. In vitro and in vivo antidiabetic activity of the leaves of. All plant extracts were administrated orally with a dose of 50 mgkg body weight for 30 consecutive days. Furthermore, pharmacological and chemical investigations are under process to find out the active constituents responsible for antidiabetic activity and to elucidate its mode of action.
This observed antidiabetic activity of this extract might be due to their phytochemical constituents previously reported. In vivo assessment of antidiabetic and antioxidant. The assay was started by addition of 4 npgp and the change in absorbance at 405nm was measured by spectrophotometer and ic 50 values were calculated 18. Many reports are available on plant lectins antidiabetic activity by possessing various mechanisms 1012. Administration of hydroethanolic leaves extract of. In vitro and in vivo antidiabetic and antioxidant activities of.
Antidiabetic activity of the hydroethanolic extract of c. Oral treatment of aetpb using rat oral needle at 100 and 200. In vivo antidiabetic activity of the ethanolic crude extract of sorbus. Data were analyzed using one way anova followed by tukeys post hoc multiple comparison test. There is no report was found on antidiabetic potential of this plant. Plant material was subjected to the extraction preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform. The phytoconstituents biomarkers present in aetpb were characterized by highperformance liquid chromatography hplc analysis. Further, in vivo antidiabetic activity was assessed in streptozotocin. The antidiabetic effects were found to be dosedependent. Nov 27, 2019 the extract was subjected to detail in vivo antidiabetic study. In vitro and in vivo evaluation of antidiabetic potential of.
Determination of in vitro antidiabetic effects of zingiber. The purpose of this study is to examine the acute toxicity and antidiabetic activity of asystacia gangetica leaf ethanol extract. The result of the present study confirmed that the methanolic extract of e. However, studies on the antihyperglycemic activity of fruitpulp of e. In vitro, glucose uptake by l6 cells was measured to determine the effect of extracts on cellular glucose uptake behaviour. The ethanol leaf extract of these plants exhibited highest in vitro antioxidant activity. Invivo antidiabetic activity of methanolic extract of.
The effect of the extract on body weight and diabetic dyslipidemia was also studied on stzinduced diabetic mice. The effect of the extract supported the traditional claim of the plant. Additionally, antioxidant activity of the plant extract was determined using 2,2diphenyl1picrylhydrazine free radical scavenging assay. This study aimed at bioscreening for hypoglycemic activity of the aqueous and organic fractions of s. In vitro antidiabetic and antioxidant activities of. In vivo animal models of diabetes mellitus diabetes can be induced by pharmacologic, surgical or genetic manipulations in. In vitro antidiabetic activity of polar and nonpolar. Evaluation of in vivo antidiabetic, antidyslipidemic and.
Rosacea ranked highly and increased the transport of. Antidiabetic herbal formulations are considered to be more effective for treatment of diabetes. The studied plant extract contained alkaloids, flavonoids. In vitro and in vivo antidiabetic activity of the leaves. In vivo animal models of diabetes mellitus diabetes can be induced by pharmacologic, surgical or genetic manipulations in several animal species.
Pdf in vivo antidiabetic activity of the aqueous leaf. In vitro and in vivo evaluation of antidiabetic potential. In vivo antidiabetic activity of the ethanolic crude extract. To assess the antidiabetic potential of extracts of selected nigerian medicinal plant recipes collected from tmps. The entire plant or its different parts can be used in the form of a whole plant, crude extract, and aqueous decoctions. The dose was selected according the toxicity study, which were safety for all doses used. Antidiabetic activity of the compounds isolated from rhus mysorensis plant extract ganji renuka rani 1,2, singara charya ma2, m. Among them, melia azedarach twigs and tanacetum nubigenum leaves extract possess strong invitro as well as invivo antidiabetic effects which may be responsible for their hypoglycemic property. In vitro antidiabetic activity and mechanism of action of. The present study was designed to evaluate the antidiabetic potential of the aqueous leaves extract of zanthoxylum armatum dc. However, due to the potential toxicity of the plant extract it must be prescribed with caution. Anti diabetic medicinal plants open access journals. Oct 26, 2018 the effect of the extract on body weight and diabetic dyslipidemia was also studied on stzinduced diabetic mice. Endophytic fungal extracts showed antidiabetic activity 7,8.
Stzinduced diabetic mice showed significant p in vitro bioassays. The ethanolic extract was more effective in reducing the blood glucose levels during acute. The previous study showed that water extract of fruitpulp had been found to show hypoglycemic activity immediately or as early as 30 minutes 4, while seeds. The selected plant extracts were studied for their effect on inhibition of glycosylation of haemoglobin and glucose transport across yeast cells. In vivo assessment of antidiabetic and antioxidative activity. This provides deep insight into the use of extracts for therapeutic purpose and. Rjpt in vivo antidiabetic and in vitro antioxidant and. In vitro and in vivo antidiabetic effect of extracts of melia azedarach. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf le and fruit fe from p. In vitro and in vivo antidiabetic activity of lectin n.
In vitro study results scientifically supported the terminalia paniculata bark in vivo antidiabetic activity. Full text evaluation of in vivo antidiabetic, antidyslipidemic and in. The in vivo antidiabetic effects of the plant were. In vivo and in vitro antidiabetic activity of terminalia. In this study, the total phenolic and flavonoid contents, the 2,2diphenyl1picryl hydrazyl dpph radical scavenging ability and the ferric reducing power frap of aloe vera were measured to determine the antioxidant activity of this species.
Evaluation of antidiabetic activity of biologically. Pdf in vivo antidiabetic activity of capparis erythrocarpos. In vitro antidiabetic activity by glucose uptake method. Phytochemical and in vitro antidiabetic activity of. Identification and quantification of bioactive compounds present was analysed by. Ajuga remota benth is traditionally used in ethiopia for the management of diabetes mellitus. Ethanolic extract of leaves revealed the presence of high phenolic 33.
Jul 14, 20 therefore, the present study was aimed to investigate in vivo antidiabetic activity of aqueous extract of terminalia paniculata bark aetpb in type 2 diabetic rats. Additionally, antioxidant activity of the plant extract was determined. Department of environmental science and technology, jntu, hyderabad 2. Glucosidase from yeast and plant extract or control drug in the range of 0. Antidiabetic activity of the compounds isolated from rhus mysorensis plant extract. In vivo antidiabetic and lipid lowering activity and in. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts sindhu. Antidiabetic activity of aqueous and methanol extract and fractions of gongronema latifolium leaves in alloxan diabetic rats p. Preclinical evaluation of antidiabetic activity of poly. In vivo antidiabetic activity of aqueous and ethyl acetate leaf extract. Okolo rural dwellers in nigeria often resort to herbal remedy and dietary control in the treatment of diabetes mellitus dm.